Several subsequent-technology sequencing and Evaluation equipment work with SAM/BAM. For customized monitor Screen, the principle advantage of indexed BAM in excess of PSL together with other human-readable alignment formats
This is accompanied by a 32-little bit number in the identical format that describes the volume of bases from the file. Following, the bases by themselves are outlined, packed two bases to the byte. The initial base is packed inside the high-buy 4 bits (nibble); the second foundation is packed within the minimal-purchase four bits:
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For a whole definition of all fields from the twoBit structure, see this description inside the supply code.
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maskBlockStarts - an assortment of length maskBlockCount of 32 little bit integers indicating the setting up place of a masked block
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Just about every goal chromosome is first translated into amino acids for every on the six diverse reading frames. The resulting protein PSL is a hybrid; the question fields are all in amino acid coordinates and measurements, though the focus on databases fields are in nucleic acid chromosome coordinates and measurements.
rightCount -- Commonly the quantity of bases in the aligning species in between the end of this alignment and the start of the subsequent a person.
Each individual microarray observe set ought to also have an involved microarrayGroups.ra configuration file more info which contains additional information regarding the info in Each and every of the arrays.